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Clinical and Laboratory Facilities and methods
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RCT provides cardio-pulmonary diagnostic measurement to support its specialist clinical trials programme and biomarker research development. We have a comprehensive array of clinical and laboratory specialist techniques performed by highly specialised physiologists, technicians and research nurses.
CLINICAL Techniques
• Single Breath Gas Transfer Factor (TLCO SB) corrected for Hb
• Lung Volumes (Helium dilution)
• Spirometry (Flow Volume Loops, Peak Expiratory Flow)
• Cardio-Pulmonary exercise testing, including stress test
• Echocardiography; including cardiac output; pulmonary pressures
• Doppler Ultrasound including measurements of vascular flow
• 64 slice CT and PET CT for non-invasive CT coronary angiography
• MRI & conventional X ray
• Airway responsiveness to a range of agents
• Inhaled allergen challenge; allergen
• Exercise challenge
• Bronchoscopy, bronchial biopsy, brushings and BAL
• Induced sputum
• Exhaled NO
• Bronchial mucosal blood flow
• Nasal challenge to allergen or LPS; nasal lavage; nasal biopsy
• Acoustic Rhinometry
• Nasal MRI (biomarker of inflammation)
LABORATORY Techniques
General
• Sputum processing and analysis
• Biopsy processing and analysis (frozen)
• BAL processing and analysis
• Immunohistochemistry
• ELISA/RIA and other colourimetric assays
• Image analysis
• Flow Cytometry
• Fluorometry
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Whole Blood assays
• measurement of markers of inflammation
• pharmacokinetics and pharmacodynamics on whole human blood
• flow cytometry: activation markers, chemotaxis, and CD11b upregulation
• chemokine levels using the Luminex bead array system
Nasal challenge assays
• nasal lavage: cytology
• nasal biopsy and brushing
• Multiple array analyses for chemokines and cytokines
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